USE OF MICROPACKED COLUMNS FOR QUANTITATIVE SFC

The performance of a type of micropacked column for the quantitative S FC analysis of samples containing solutes at very different concentrat ions has been studied. The chromatographic characteristics of the colu mn were compatible with high sample volume loadability, and at the sam e time adequate efficiency and low pressure drop. In order to increase both the separating power and sample capacity of the column, the perc entage liquid phase loading was optimized. The accuracy, precision, an d detection limit obtainable with the micropacked column are compared with the performance of a wall coated open tubular capillary column ha ving the same phase ratio.