MATHEMATICAL-MODELING AND PARAMETRIC STUDIES OF AFFINITY-CHROMATOGRAPHY

Authors
CARLSSON F AXELSSON A ZACCHI G
Citation
F. Carlsson et al., MATHEMATICAL-MODELING AND PARAMETRIC STUDIES OF AFFINITY-CHROMATOGRAPHY, Computers & chemical engineering, 18, 1994, pp. 190000657-190000661
Citations number
9
Categorie Soggetti
Computer Application, Chemistry & Engineering","Engineering, Chemical","Computer Science Interdisciplinary Applications
Journal title
Computers & chemical engineering → ACNP
ISSN journal
00981354
Volume
18
Year of publication
1994
Supplement
S
Pages
190000657 - 190000661
Database
ISI
SICI code
0098-1354(1994)18:<190000657:MAPSOA>2.0.ZU;2-Y
Abstract
In this study a model based on mass transfer and sorption rate constan ts with physical significance is used for simulation of affinity chrom atography, The simulation program has been used to perform a parametri c analysis of the adsorption of lysozyme on Cibacron Blue-Sepharose CL -6B. The influence of process parameters as well as physical parameter s on the chromatography process was investigated. The external and the internal mass transfer as well as the sorption rate were found to con tribute to the control of the overall rate of the system. The most imp ortant process parameter was the radius of the beads. The optimum bead size was found to be around 50 mu m.