A. Rahmat et al., EFFECT OF GAMMA-TOCOTRIENOL AND ALPHA-TOCOPHEROL ON BLOOD GLUTATHIONEAND TUMOR-MARKER ENZYMES DURING CHEMICAL HEPATOCARCINOGENESIS IN THE RAT, Journal of clinical biochemistry and nutrition, 15(3), 1993, pp. 195-202
The protective effect of two types of vitamin E (a-tocopherol and gamm
a-tocotrienol) in rats treated with diethylnitrosamine (DEN) and 2-ace
tylaminofluorene (AAF) were studied by determination of plasma alkalin
e phosphatase (ALP), plasma and liver microsomal gamma-glutamyl transp
eptidase (GGT) activities, and blood glutathione (GSH). Rats treated w
ith DEN/AAF had significantly elevated plasma and microsomal GGT, plas
ma ALP activities, and blood GSH levels compared with the normal contr
ols (p<0.05). Supplementation with vitamin E of normal controls did no
t affect the enzyme activities or blood GSH. In rats treated with DEN/
AAF, vitamin E supplementation attenuated GGT and ALP activities and b
lood GSH levels. The optimum dose required for highest attenuation of
the tumor marker enzyme activities was 34 mg/kg diet for alpha-tocophe
rol and 30 mg/kg diet for gamma-tocotrienol. Higher doses of the vitam
in did not show further attenuation in the level of the tumor marker e
nzyme activities.