COVALENT IMMOBILIZATION OF BETA-GALACTOSIDASE ON CORN GRITS - A SYSTEM FOR LACTOSE HYDROLYSIS WITHOUT DIFFUSIONAL RESISTANCE

Authors
SISO MIG FREIRE A RAMIL E BELMONTE ER TORRES AR CERDAN E
Citation
Mig. Siso et al., COVALENT IMMOBILIZATION OF BETA-GALACTOSIDASE ON CORN GRITS - A SYSTEM FOR LACTOSE HYDROLYSIS WITHOUT DIFFUSIONAL RESISTANCE, Process biochemistry, 29(1), 1994, pp. 7-12
Citations number
22
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
Journal title
Process biochemistry → ACNP
ISSN journal
13595113
Volume
29
Issue
1
Year of publication
1994
Pages
7 - 12
Database
ISI
SICI code
1359-5113(1994)29:1<7:CIOBOC>2.0.ZU;2-#
Abstract
Beta-Galactosidase from Saccharomyces fragilis was covalently linked b y glutaraldehyde to chemically modified corn grits, an inexpensive mat erial with good mechanical properties for use in bioreactors. The immo bilized enzyme was used to hydrolyse milk-whey lactose in a recycling packed-bed bioreactor. Quantification of external and internal diffusi onal resistances showed that the reaction rate was not limited by diff usion.