RETROSPECTIVE STUDY OF PROGNOSTIC VALUE OF DNA-PLOIDY AND PROLIFERATIVE ACTIVITY IN NEUROBLASTOMA

Citation
Sn. Huddart et al., RETROSPECTIVE STUDY OF PROGNOSTIC VALUE OF DNA-PLOIDY AND PROLIFERATIVE ACTIVITY IN NEUROBLASTOMA, Journal of Clinical Pathology, 46(12), 1993, pp. 1101-1104
Citations number
14
Categorie Soggetti
Pathology
ISSN journal
00219746
Volume
46
Issue
12
Year of publication
1993
Pages
1101 - 1104
Database
ISI
SICI code
0021-9746(1993)46:12<1101:RSOPVO>2.0.ZU;2-O
Abstract
Aim-To assess the prognostic value of age and stage at diagnosis, site of primary tumour, cell ploidy and N-myc copy number in children with neuroblastoma. Methods-Flow cytometry was used to determine the cellu lar DNA content of paraffin wax embedded archival material from 69 cas es of neuroblastoma and was successful in 52. Results-The age, stage, and survival distribution of the sampled cases was not significantly d ifferent from that in a larger population based series. There were sev en diploid (''non-aneuploid'') and 45 aneuploid (including two tetrapl oid and four triploid) tumours. The 10 year survival was significantly better for cases of aneuploid rather than diploid tumours (p < 0.05). An important new finding was that 10 year survival was also significa ntly better for tumours with a low percentage of cells in S phase (p < 0.03). Conclusion-The percentage of cells in S phase, a measure of th e proliferative activity of the tumour, correlated with prognosis in n euroblastoma. This should be measured with other biological features o f the disease, such as N-myc copy number, when prognostic indicators a re being assessed.