P. Jadubansa et al., PHYSIOLOGY OF PRODUCTION OF VIABLE BIOMASS AND SPORE INOCULUM FOR THEBIOCONTROL AGENT IDRIELLA (MICRODOCHIUM) BOLLEYI, Enzyme and microbial technology, 16(1), 1994, pp. 24-28
Biomass of the biocontrol agent Idriella bolleyi can be produced in su
bmerged liquid culture as mycelium, conidia, and chlamydospores. Each
fraction was separated and quantified. The conidial fraction appeared
to be the most thermotolerant, suggesting that this form had the best
potential shelf life of the three. Conidia were produced during the ea
rly phase of growth and their production did not appear to require any
form of nutrient exhaustion or growth rate decay. The highest viabili
ty per unit biomass was obtained in carbon-limited medium, with sodium
nitrate as the nitrogen source, after 6 h. Maximum biomass was not ob
tained in this medium until 167 h and, as the biocontrol product is li
kely to be sold by weight, an acceptable compromise between the two pa
rameters must be found. Excess ammonium chloride in the medium appeare
d to inhibit growth. A procedure, involving culture homogenization, wa
s adopted in order to estimate potential viability in shake-flask liqu
id culture.