PLASMA-LEVELS OF BACTERICIDAL PERMEABILITY-INCREASING PROTEIN (BPI) AND LIPOPOLYSACCHARIDE-BINDING PROTEIN (LBP) DURING HEMODIALYSIS

Several proteins modify the biological response to lipopolysaccharide (LPS). Both bactericidal/permeability-increasing factor (BPI), a prote in stored in neutrophils, and the acute phase protein LPS-binding prot ein (LBP) bind to LPS; however, BPI inhibits while LBP enhances bindin g of LPS to leukocytes and subsequent induction of cytokines. We inves tigated plasma levels of BPI, LBP, elastase and C5a before, during and after hemodialysis (HD). Six patients were dialysed with Cuprophane(R ) (Cup) and polysulfone (PS) low-flux dialyzers on two consecutive HD sessions. There was a significant, 10.9 +/- 2.8-fold increase in BPI a fter 4-hour HD compared to predialysis and a 4.4 +/- 1.6-fold increase in elastase after 4-hour HD using Cup. Plasma levels of BPI and elast ase decreased rapidly after the dialysis session. HD with PS resulted in a smaller, but still significant rise in BPI (3.7 +/- 1.6-fold at 4 hours) and elastase (1.69 +/- 0.2-fold at 4 hours). Levels for BPI an d elastase were similar in the arterial and venous blood lines of the dialyzer. Plasma levels of LBP did not change during or after the HD s ession. These data indicate that BPI, but not LBP is released during H D with Cup and to a lesser extent with PS. Activation of neutrophils a nd release of BPI during HD may influence the biological response to b acterial products possibly introduced during HD.