Representatives of 21 species of the basidiomycete Agaricus were exami
ned for the presence of the linear mitochondrial plasmid, pEM, and mit
ochondrial sequences that hybridize to the pEM RNA polymerase gene. pE
M-like plasmids were detected in only two species although other types
of plasmids were detected in eight species. pEM RNA polymerase-like s
equences were detected in the mitochondrial DNA of 20 species. A commo
n sequence ancestor to the pEM plasmid and to the mitochondrial RNA po
lymerase-like sequences probably existed in the Agaricus mitochondrial
ancestor prior to speciation of the genus.