A 3667-base pair (bp) fragment of the mitochondrial genome of the crus
tacean Daphnia pulex has been sequenced and found to contain the compl
ete genes for the small subunit ribosomal RNA, ND2, seven tRNAs and th
e control region. This organization is identical to that found in Dros
ophila yakuba mtDNA yet D. pulex mtDNA exhibits several unique feature
s when compared to other mitochondrial sequences. The sequenced fragme
nt is only 62.6% A + T which is much lower than that of any other arth
ropod mtDNA sequenced to date. D. pulex mtDNA also exhibits length con
servation having shorter coding and non-coding regions. The putative c
ontrol region is 689 bp in length and includes a sequence that has the
potential to fold into a hairpin structure with a perfect 20-bp pair
stem and a 22-base loop.