High performance liquid chromatography with electrochemical detection
(HPLC-EC) was used to measure 8-hydroxy-2'-deoxyguanosine (8-OHdG), a
marker for oxidative DNA damage, in mammary gland isolated from tumor-
bearing and tumor-free rats fed diets of varied fatty acid composition
and vitamin E and selenium content. A method for tissue preparation a
nd analysis is reported and a significant positive correlation shown b
etween degree of unsaturation of dietary fatty acids and 8-OHdG concen
tration, regardless of antioxidant status. The increase in 8-OHdG conc
entration with greater fatty acid unsaturation was more pronounced in
the absence of adequate dietary vitamin E and selenium. The implicatio
ns of these data for defining the role of dietary lipid in the process
of mammary carcinogenesis are discussed.