ADVANCEMENT OF THE DNA-FINGERPRINTING BY MEANS OF MOLECULAR HYBRIDIZATION WITH MODIFIED OLIGONUCLEOTIDES, FORMING THE DUPLEXES WITH INCREASED STABILITY

Authors
PROSNYAK MI VESELOVSKAYA SI MYASNIKOV VA EFREMOVA EY POTAPOV VK LIMBORSKAYA SA SVERDLOV ED
Citation
Mi. Prosnyak et al., ADVANCEMENT OF THE DNA-FINGERPRINTING BY MEANS OF MOLECULAR HYBRIDIZATION WITH MODIFIED OLIGONUCLEOTIDES, FORMING THE DUPLEXES WITH INCREASED STABILITY, Bioorganiceskaa himia, 19(9), 1993, pp. 917-919
Citations number
8
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Bioorganiceskaa himia → ACNP
ISSN journal
01323423
Volume
19
Issue
9
Year of publication
1993
Pages
917 - 919
Database
ISI
SICI code
0132-3423(1993)19:9<917:AOTDBM>2.0.ZU;2-E
Abstract
The modified oligodeoxyribonucleotide (m5C-n2A-e5C)5 containing 5-meth ylcytosine and 2-aminoadenine instead of cytosine and adenine residues , respectively has been used as a hybridisation probe in DNA fingerpri nting. The oligonucleotide, due to the substitutions forms more stable duplexes, with complementary sequence in DNA than the corresponding n onmodified pentadecanucleotide. The comparison with its natural counte rpart displays considerably increased intensity of bands in patterns o btained with modified analog. The use of such analogues can increase s ensitivity and shorten time of DNA fingerprinting.