INTERACTION OF OUTER-MEMBRANE PROTEIN FRO M YERSINIA-PSEUDOTUBERCULOSIS WITH LIPID-A AND ITS SYNTHETIC ANALOGS

Authors
FEDOREYEVA LI GORBACH VI
Citation
Li. Fedoreyeva et Vi. Gorbach, INTERACTION OF OUTER-MEMBRANE PROTEIN FRO M YERSINIA-PSEUDOTUBERCULOSIS WITH LIPID-A AND ITS SYNTHETIC ANALOGS, Bioorganiceskaa himia, 19(10), 1993, pp. 933-940
Citations number
22
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Bioorganiceskaa himia → ACNP
ISSN journal
01323423
Volume
19
Issue
10
Year of publication
1993
Pages
933 - 940
Database
ISI
SICI code
0132-3423(1993)19:10<933:IOOPFM>2.0.ZU;2-X
Abstract
Interaction of the major outer membrane protein form Yersinia pseudotu berculosis with lipid A was investigated by intrinsic fluorescence, CD spectroscopy and CsCl gradient centrifugation methods. The protein wa s shown to have two independent binding sites with an association cons tant 6,1.10(4) M-1. The interaction depends on both the type of the gl ycoside bond and hydrophylic - hydrophobic balance of the glycolipid m olecule.