PLASMID PATTERNS OF BACILLUS-THURINGIENSIS 81 AND ITS CRYSTAL-NEGATIVE MUTANTS

Citation
P. Afkhami et al., PLASMID PATTERNS OF BACILLUS-THURINGIENSIS 81 AND ITS CRYSTAL-NEGATIVE MUTANTS, Biotechnology letters, 15(12), 1993, pp. 1247-1252
Citations number
15
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
ISSN journal
01415492
Volume
15
Issue
12
Year of publication
1993
Pages
1247 - 1252
Database
ISI
SICI code
0141-5492(1993)15:12<1247:PPOB8A>2.0.ZU;2-L
Abstract
A local isolate of Bacillus thuringiensis (B.t. 81) contained five dis tinct plasmids Relative amounts of these plasmids varied at different stages of growth.Two Cry- mutants of the strain maintained all plasmid s. The mutants, however, exhibited some variations in the patterns of large plasmids depending on the composition of culture medium even tho ugh they failed to produce crystal protein. Restriction analysis of th e plasmids was also carried out for future cloning studies.