APPLICATION OF 6-AMINOHEXYLATED MACROPORO US GLUCOMANNAN GEL TO HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FOR PURIFICATION OF COAGULATION FACTOR-VIII

Citation
M. Yamaguchi et al., APPLICATION OF 6-AMINOHEXYLATED MACROPORO US GLUCOMANNAN GEL TO HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FOR PURIFICATION OF COAGULATION FACTOR-VIII, Nippon kagaku kaishi, (12), 1993, pp. 1335-1348
Citations number
16
Categorie Soggetti
Chemistry
Journal title
ISSN journal
03694577
Issue
12
Year of publication
1993
Pages
1335 - 1348
Database
ISI
SICI code
0369-4577(1993):12<1335:AO6MUG>2.0.ZU;2-4
Abstract
6-Aminohexylated macroporous glucomannan gel beads (AH-GM gel) have be en developed and applied to chromatographic separation of blood coagul ation factor VIII(FVIII) in plasma cryoprecipitate fraction. Retained capacity of FVIII on various kinds of AH-spherical gels was measured b y a batch-wise method and was apparently dependent on exclusion limit (Mlim) of the gels. AH-GM gel (MliM 1 x 10(7)) possessed the capacity at least 10 fold higher than EAH Sepharose(R) 4 B. The HPLC system usi ng an AH-GM gel column also showed strong retention and good elution r ecovery of FVIII under the operational conditions at 0.5-4 m/h flow ra te.