Amo. Brett et al., ELECTROCHEMICAL DETERMINATION OF CARBOPLATIN IN SERUM USING A DNA-MODIFIED GLASSY-CARBON ELECTRODE, Electroanalysis, 8(11), 1996, pp. 992-995
An electrochemical method for the determination of the anticancer plat
inum drug carboplatin using a DNA-modified electrode was developed. Th
is electrode was successfully used for the electrochemical determinati
on of carboplatin in serum samples of patients with ovarian cancer und
ergoing treatment. The electrochemical results clearly demonstrated th
at, for low concentrations, carboplatin interacts preferentially with
adenine rather than guanine groups in the DNA and they can contribute
to clarifying the mechanisms of interaction of platinum anticancer dru
gs with DNA. The pharmacokinetics corresponding to the administration
of the drug was followed electrochemically and the detection limit in
serum samples was 5.7 x 10(-6)M. Due to a similar mechanism of reactio
n with DNA other platinum anticancer drugs can be determined by this m
ethod.