ELECTROCHEMICAL DETERMINATION OF CARBOPLATIN IN SERUM USING A DNA-MODIFIED GLASSY-CARBON ELECTRODE

An electrochemical method for the determination of the anticancer plat inum drug carboplatin using a DNA-modified electrode was developed. Th is electrode was successfully used for the electrochemical determinati on of carboplatin in serum samples of patients with ovarian cancer und ergoing treatment. The electrochemical results clearly demonstrated th at, for low concentrations, carboplatin interacts preferentially with adenine rather than guanine groups in the DNA and they can contribute to clarifying the mechanisms of interaction of platinum anticancer dru gs with DNA. The pharmacokinetics corresponding to the administration of the drug was followed electrochemically and the detection limit in serum samples was 5.7 x 10(-6)M. Due to a similar mechanism of reactio n with DNA other platinum anticancer drugs can be determined by this m ethod.