PURIFICATION OF ALKALINE PROTEASE OF RHIZOPUS-ORYZAE BY FOAM FRACTIONATION

Studies on the cost effective purification method is very much essenti al for the industrially important enzyme like protease. As foam fracti onation is an important technique in downstream processing of the biol ogicals, purification of the crude proteolytic enzyme produced by Rhiz opus oryzae has been attempted using converging-diverging foam fractio nator. The effects of different parameters studied on the purification efficiency by foam fractionation were pH of the broth, air flow rate, initial liquid height. The results obtained were found quite encourag ing at pH 10 with 1 LPM of air flow rate at low initial liquid height.