PROCESS OF PENICILLIN-G HYDROLYSIS CATALYZED BY PENICILLIN ACYLASE IMMOBILIZED ON ACRYLIC CARRIER

The usefulness of penicillin acylase immobilized onto butyl acrylate - ethyl glycol dimethacrylate (called in this paper acrylic carrier) in penicillin G hydrolysis performed in a stirred tank reactor is shown. The enzyme-acrylic carrier preparation does not deteriorate its own p roperties in the mixing condition of slurry reactor. The experiments w ere carried out in a batch and a continuous stirred tank reactor as we ll as continuous stirred tank reactors in series. It was found to be a satisfactory agreement between experimental and predicted results. It also indicated the optimal substrate concentration range which provid es the most effective enzyme operation. A superiority of the three rea ctors in series over the batch reactor is shown.