G. Alemany et al., THIN-LAYER CHROMATOGRAPHIC DETERMINATION OF BRAIN CATECHOLAMINES AND 5-HYDROXYTRYPTAMINE, BMC. Biomedical chromatography, 7(6), 1993, pp. 315-316
A simple and sensitive (up to nanogram level) method to determine nore
pinephrine, serotonine and dopamine in rat brain is described. The ami
nes are acetyled and the derivatives are resolved by thin layer chroma
tography (TLC) on silica high performance thin layer chromatography (H
PLC) plates. Quantification is achieved by fluorescence densitometry a
t 415 nm excitation wavelength.