THIN-LAYER CHROMATOGRAPHIC DETERMINATION OF BRAIN CATECHOLAMINES AND 5-HYDROXYTRYPTAMINE

Authors
ALEMANY G NICOLAU MC GAMUNDI A RIAL R
Citation
G. Alemany et al., THIN-LAYER CHROMATOGRAPHIC DETERMINATION OF BRAIN CATECHOLAMINES AND 5-HYDROXYTRYPTAMINE, BMC. Biomedical chromatography, 7(6), 1993, pp. 315-316
Citations number
7
Categorie Soggetti
Chemistry Analytical","Pharmacology & Pharmacy",Biology
Journal title
BMC. Biomedical chromatography → ACNP
ISSN journal
02693879
Volume
7
Issue
6
Year of publication
1993
Pages
315 - 316
Database
ISI
SICI code
0269-3879(1993)7:6<315:TCDOBC>2.0.ZU;2-A
Abstract
A simple and sensitive (up to nanogram level) method to determine nore pinephrine, serotonine and dopamine in rat brain is described. The ami nes are acetyled and the derivatives are resolved by thin layer chroma tography (TLC) on silica high performance thin layer chromatography (H PLC) plates. Quantification is achieved by fluorescence densitometry a t 415 nm excitation wavelength.