Gw. Sandker et al., MAINTENANCE OF VIABILITY AND TRANSPORT FUNCTION AFTER PRESERVATION OFISOLATED RAT HEPATOCYTES IN VARIOUS SIMPLIFIED UNIVERSITY-OF-WISCONSIN SOLUTIONS, Biochemical pharmacology, 46(11), 1993, pp. 2093-2096
Rat hepatocytes were preserved for 24hr with high recovery and good ma
intenance of viability and transport function both in University of Wi
sconsin (UW) solution and in various simplified UW solutions. Cell qua
lity is somewhat affected after 48 hr of preservation in both the orig
inal UW solution and the simplified solutions. ATP content and uptake
rate of taurocholic acid are more sensitive markers of cell viability
than Trypan blue exclusion or the MTT test. A much less expensive solu
tion than UW, containing only K+-lactobionate, KH2PO4, MgSO4 and raffi
nose, can be used successfully for preservation of rat hepatocytes for
24 hr for drug transport studies.