H. Sugaya et al., ANTIGEN-DEPENDENT RELEASE OF INTERLEUKIN-5 IN-VITRO FROM SPLEEN-CELLSOF MICE INFECTED WITH ANGIOSTRONGYLUS-CANTONENSIS, International journal for parasitology, 23(7), 1993, pp. 865-869
Antigen dependent release of interleukin 5 in vitro from spleen cells
of mice infected with Angiostrongylus cantonensis. International Journ
al for Parasitology 23: 865-869. The kinetics of eosinophil growth and
/or survival stimulating factors (Eo-stimulating factors) released by
spleen cells from A. cantonensis-infected mice were assessed by in vit
ro marrow cultures. When spleen cells from C57BL/6 mice 8 to 20 days p
.i. were cultured with young adult A. cantonensis worm antigen or Con
A, almost equal amounts of Eo-stimulating factors were detected in the
conditioned media obtained from both stimulations. No Eo-stimulating
factor activity was detected in cultures from spleen cells without sti
mulation or from normal spleen cells with stimulation. Production of E
o-stimulating factors was inhibited by the pretreatment of the spleen
cells with anti-Thy 1.2 or anti-L3T4 antibodies plus complement but no
t with anti-Lyt2.2 antibody. In the presence of anti-mouse IL-5 monocl
onal antibody, the activity of Eo-stimulating factors was inhibited by
up to 99%. IL-5, therefore, appears to play a principal role in induc
tion of eosinophilia in mice infected with A. cantonensis.