PCR FINGERPRINTING CRYPTOCOCCUS-NEOFORMANS BY RANDOM AMPLIFICATION OFPOLYMORPHIC DNA

Citation
Ac. Crampin et al., PCR FINGERPRINTING CRYPTOCOCCUS-NEOFORMANS BY RANDOM AMPLIFICATION OFPOLYMORPHIC DNA, Journal of medical and veterinary mycology, 31(6), 1993, pp. 463-465
Citations number
9
Categorie Soggetti
Mycology
ISSN journal
02681218
Volume
31
Issue
6
Year of publication
1993
Pages
463 - 465
Database
ISI
SICI code
0268-1218(1993)31:6<463:PFCBRA>2.0.ZU;2-D
Abstract
A discriminating fingerprinting system is described for Cryptococcus n eoformans isolates in which random amplification of polymorphic DNA by the polymerase chain reaction is achieved with a single short primer, 5'-GCGCACGG-3'. DNA from 12 isolates was examined and gave rise to 12 distinct fingerprints.