L. Baldassarri et al., VARIABLE FIXATION OF STAPHYLOCOCCAL SLIME BY DIFFERENT HISTOCHEMICAL FIXATIVES, European journal of clinical microbiology & infectious diseases, 12(11), 1993, pp. 866-868
A variety of histochemical fixatives were used to compare the fixation
of bacterial films produced by a standard slime-producing strain of S
taphylococcus epidermidis on plastic tissue culture plates. Some reage
nts were completely ineffective in fixing the slime layer, whereas oth
ers gave variable results. The best alternative to the fixative of the
reference method, the potentially explosive Bouin's reagent, was air
drying.