BINDING CHARACTERISTICS OF RECOMBINANT HUMAN ENDOTHELIN RECEPTOR-ET(A) AND RECEPTOR-ET(B) EXPRESSED IN BABY HAMSTER-KIDNEY CELLS

Authors
HECHLER U BECKER A HAENDLER B SCHLEUNING WD
Citation
U. Hechler et al., BINDING CHARACTERISTICS OF RECOMBINANT HUMAN ENDOTHELIN RECEPTOR-ET(A) AND RECEPTOR-ET(B) EXPRESSED IN BABY HAMSTER-KIDNEY CELLS, Journal of cardiovascular pharmacology, 22, 1993, pp. 190000015-190000017
Citations number
15
Categorie Soggetti
Cardiac & Cardiovascular System","Respiratory System","Pharmacology & Pharmacy
Journal title
Journal of cardiovascular pharmacology → ACNP
ISSN journal
01602446
Volume
22
Year of publication
1993
Supplement
8
Pages
190000015 - 190000017
Database
ISI
SICI code
0160-2446(1993)22:<190000015:BCORHE>2.0.ZU;2-9
Abstract
Expression constructs for human endothelin (ET) receptors ET(A) and ET (B) were made by subcloning the corresponding coding sequences into th e pMPSV/CMV vector. They were used together with plasmids bearing resi stances to puromycin and hygromycin for transfection of baby hamster k idney cells. Culture in the presence of both antibiotics allowed the s election of cell lines stably expressing one receptor or the other. Co mpetitive binding experiments using ET-1 and ET-3 showed the typical i sopeptide-selective and non-isopeptide-selective profiles for recombin ant ET(A) and ET(B), respectively.