Z. Racz et C. Baroti, TECHNICAL ASPECTS OF BUFFY COAT REMOVAL FROM WHOLE-BLOOD AND THOSE OFPLATELET PRODUCTION FROM SINGLE BUFFY COAT UNITS, Biomedizinische Technik, 38(11), 1993, pp. 266-269
Technical aspects of buffy coat removal and those of platelet producti
on from the buffy coat were studied using the so-called Amsterdam tech
nique. A long stomach clamp physically separates the buffy coat from e
rythrocyte concentrate. A better removal of platelets and leukocytes w
as obtained when blood stored overnight was processed vs. fresh blood.
As 71.4 +/- 16.4 % of leukocytes was removed from blood stored overni
ght, it resulted in a mean leukocyte content of 0.84 +/- 0.52.10(9) of
the erythrocyte concentrate. The haematocrit of the single buffy coat
units have an essential impact on platelet production from buffy coat
. A haematocrit of less than 30 % seems to be preferable with a volume
over 100 ml. The leukocyte contamination of the platelet concentrate
is less when it is produced from blood stored overnight.