Aw. Duckworth et al., CONSTRUCTION OF INTEGRATING DERIVATIVES OF PHV60 FOR USE IN LACTOBACILLUS-PLANTARUM, FEMS microbiology letters, 114(3), 1993, pp. 267-272
Lactobacillus plantarum plays an important role in a wide range of foo
d and agricultural fermentations worldwide. In recent years, there has
been substantial interest in the development of genetically manipulat
ed strains of Lb. plantarum, which possess improved characteristics fo
r use in these fermentations. If these genetically manipulated strains
are to be approved for extensive commercial use, permission to use th
em is more likely to be granted if the recombinant genetic characteris
tics are introduced into the host chromosome. This paper describes the
behaviour of plasmid pHV60 in Lactobacillus plantarum NCDO1193, and d
emonstrates that it can act as convenient small integrative vector in
the organism.