RAPID PREPARATION OF DNA FROM PHYTOPATHOGENIC MYCOPLASMA-LIKE ORGANISMS FOR POLYMERASE CHAIN-REACTION ANALYSIS

Authors
FIRRAO G LOCCI R
Citation
G. Firrao et R. Locci, RAPID PREPARATION OF DNA FROM PHYTOPATHOGENIC MYCOPLASMA-LIKE ORGANISMS FOR POLYMERASE CHAIN-REACTION ANALYSIS, Letters in applied microbiology, 17(6), 1993, pp. 280-281
Citations number
5
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
17
Issue
6
Year of publication
1993
Pages
280 - 281
Database
ISI
SICI code
0266-8254(1993)17:6<280:RPODFP>2.0.ZU;2-H
Abstract
A simple procedure for the processing of MLO (mycoplasma-like organism s)-infected plant samples for PCR analysis is described. It is based o n differential centrifugation and removal of enzyme inhibiting agents with the aid of a resin. Results of the amplifications are comparable with those achieved with more complex DNA extraction procedures. The m ethod does not require any extraction with organic solvents or alcohol ic precipitation of nucleic acids.