THE EFFECT OF COLONY AGE ON PCR FINGERPRINTING

Authors
COUTINHO HLC HANDLEY BA KAY HE STEVENSON L BERINGER JE
Citation
Hlc. Coutinho et al., THE EFFECT OF COLONY AGE ON PCR FINGERPRINTING, Letters in applied microbiology, 17(6), 1993, pp. 282-284
Citations number
10
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
17
Issue
6
Year of publication
1993
Pages
282 - 284
Database
ISI
SICI code
0266-8254(1993)17:6<282:TEOCAO>2.0.ZU;2-K
Abstract
The pattern of DNA fragments produced by the polymerase chain reaction using arbitrary primers is strongly influenced by the age of the colo nies sampled. We show that the number, clarity and reproducibility of the bands produced is optimal when very young colonies are sampled.