Zg. Weinberg et al., THE EFFECT OF APPLYING LACTIC-ACID BACTERIA AT ENSILING ON THE AEROBIC STABILITY OF SILAGES, Journal of Applied Bacteriology, 75(6), 1993, pp. 512-518
The effect of applying commercial lactic acid bacteria inoculants at e
nsiling on the aerobic stability of silages was studied under laborato
ry conditions. The silages used were wheat, hedysarum, corn and variou
s sorghum cultivars at various stages of maturity. Three inoculants we
re used, two containing Lactobacillus plantarum, Enterococcus faecium
and Pediococcus acidilactici (H/M F, Medipharm, USA and Sil-All, Allte
ch, UK) and one containing Ent. faecium (Lacticil, M74, Medipharm, Swe
den). The inoculants were applied at 0.5 x 10(6) cfu g(-1). Silages wi
th no additives served as controls. After treatment, the chopped forag
es were ensiled in 1.51 anaerobic jars; there were six jars per treatm
ent. After ensiling for 45 d, the silages were tested for aerobic stab
ility in a test in which CO2 production was measured along with chemic
al and microbiological parameters. The inoculated silages that spoiled
upon aerobic exposure faster than the controls were those of wheat an
d of the sorghum cultivar FS5 at the milk stage of maturity. This was
evident from intensive CO2 production and development of yeasts and mo
ulds. Regression analysis indicated that aerobic deterioration of inoc
ulated silages was associated with high levels of residual water-solub
le carbohydrates and lactic acid and lack of volatile fatty acids. Aer
obic spoilage of inoculated silages was attributed mainly to yeast act
ivity.