APPLICATION OF DNA PROFILING TO PATERNITY TESTING DURING EARLY-PREGNANCY

Four probes that recognize D2S44, D17S79, D4S163 and D18S27 loci, were used to produce DNA profiles of 8- to 10-week-old chorionic villus sa mples and from blood samples of their respective parents. A total of 3 0 pedigrees were analyzed. The results showed strict Mendelian inherit ance. The cumulative paternity index (PI) and probability of paternity (W) obtained with D2S44, D17S79 and D4S163 loci were sufficient to ex ceed the required standard (PI > 369, W > 99.73) for assertion of pate rnity for all predigrees tested. Therefore, these three loci are likel y to satisfy the paternity test requirements in the majority of patern ity cases, involving unrelated individuals, in the Chinese Han populat ion. The other combinations of three loci did not produce high enough values for all cases, but all W values were > 98%. The results show th at, using DNA from chorionic villi, these polymorphic loci can resolve cases of disputed paternity during early pregnancy.