N-6-DIALKYLFORMAMIDINE-2'-DEOXYADENOSINE PHOSPHORAMIDITES IN OLIGODEOXYNUCLEOTIDE SYNTHESIS - RAPID DEPROTECTION OF OLIGODEOXYNUCLEOTIDES

Authors
THEISEN P MCCOLLUM C ANDRUS A
Citation
P. Theisen et al., N-6-DIALKYLFORMAMIDINE-2'-DEOXYADENOSINE PHOSPHORAMIDITES IN OLIGODEOXYNUCLEOTIDE SYNTHESIS - RAPID DEPROTECTION OF OLIGODEOXYNUCLEOTIDES, Nucleosides & nucleotides, 12(10), 1993, pp. 1033-1046
Citations number
28
Categorie Soggetti
Biology
Journal title
Nucleosides & nucleotides → ACNP
ISSN journal
07328311
Volume
12
Issue
10
Year of publication
1993
Pages
1033 - 1046
Database
ISI
SICI code
0732-8311(1993)12:10<1033:NPIO>2.0.ZU;2-6
Abstract
A series of dialkylformamidine protected deoxyadenosine phosphoramidit es were prepared for automated, solid-support DNA synthesis. The set o f A(bz), G(dmf), C-bz, T phosphoramidites gave high purity and high yi eld oligodeoxynucleotides, with complete deprotection at 65 degrees C in one hour. Different times and temperatures of exposure to concentra ted ammonium hydroxide were examined to establish the optimum conditio ns for deprotection of oligodeoxynucleotides.