EVIDENCE OF DISSOCIATION BETWEEN DOXORUBICIN BINDING AND CYTOTOXICITYIN CERVIX CANCER-CELLS

The relationship between cellular uptake, efflux, binding and cytotoxi city of doxorubicin (dox) has been studied in cultured human cervix ca ncer cells (MS 751) under pharmacokinetic steady state conditions. One high affinity low capacity binding site was discovered at extracellul ar dox concentrations below 10-(10)M, however, none of the intrinsic a ctivities measured after 30 hours drug exposure were correlated with t his binding site. At higher extacellular dox concentrations a non-satu rable binding of dox was dominating.