A. Beautelafitte et al., SCHIZOGONY OF PLASMODIUM-YOELII NIGERIENS IS - ROLE OF THE LATENT MEROZOITES, Annales de Parasitologie humaine et comparee, 68(5-6), 1993, pp. 211-219
Several procedures were employed to try to specify the schizogonic cyc
le of Plasmodium yoelii nigeriensis. The Percoll-glucose gradient tech
nique for concentrating the very young stages (rings and young trophoz
oites), allowing a very precise follow up of the development of the pa
rasitaemia during the first schizogonic cycles. A method for studying
the prepatencies, providing an approximation of the number of merozoit
es inoculated. A comparison between the numbers of merozoites present
in the blood, after - 1stly simple dilutions in saline, revealing the
total number of merozoites, - 2ndly dilutions in saline after a passag
e of a few hours in the organism of a mouse, revealing the number of l
atent merozoites. It was shown that the infection, during the first tw
o cycles, varies according to the time of inoculation. In all cases th
e increase of the parasitaemia occured mainly from 00:01 to 06:00. Thi
s increase of parasitaemia in mice inoculated with the Percoll concent
rated parasites was significantly high during the first cycle in mice
inoculated at 06:00 and 09:00 and during the second cycle in those ino
culated at 12:00, 15:00 and 18:00. However, differences were rapidly c
ompensated and parasitaemias became comparable at the 3rd or 4th cycle
when they ceased to be dependent on the time of inoculation.