REACTIVITIES OF 20 ANTI-HUMAN MONOCLONAL-ANTIBODIES WITH LEUKOCYTES FROM 10 DIFFERENT ANIMAL SPECIES

Twenty commercially available monoclonal antibodies (mAbs) raised agai nst various human leucocyte surface antigens were tested on lymphocyte s, monocytes and granulocytes from horse, pig, cow, sheep, goat, dog, mink, rabbit, rhesus monkey as well as man. Eight antibodies reacted w ith leucocytes from some of the animals. These were the antibodies aga inst CD2, CD4, CD5, CD8, CD14, CD18, HLA-DR, and the HLA-ABC antigen. The CD18 antibody reacted with lymphocytes, monocytes and granulocytes from all animals tested except goat. The CD14 antibody reacted viiith monocytes from pig, cow, sheep, goat, dog, mink, rabbit and monkey an d with granulocytes from pig and rabbit. The anti-HLA-ABC reacted with leucocytes from monkey and cow. Finally, the CD2, CD4, CD5 and CD8 an tibodies reacted with leucocytes from monkey only.