M. Grung et S. Liaaenjensen, ALGAL CAROTENOIDS 52 - SECONDARY CAROTENOIDS OF ALGAE-3 - CAROTENOIDSIN A NATURAL BLOOM OF EUGLENA-SANGUINEA, Biochemical systematics and ecology, 21(8), 1993, pp. 757-763
Quantitative carotenoid analysis of a natural bloom of Euglena sanguin
ea Ehrenberg revealed the presence of beta,beta-carotene (1% of total
carotenoids), monoesters of adonirubin (3%), diesters of (3S, 3'R)-ado
nixanthin (13%), diesters of (3S, 3'S)-astaxanthin (75%), 19-monoester
of (3R, 3'R, 6R)-loroxanthin (1%), (3R, 3R)-diatoxanthin (6%), diadin
oxanthin (1%) and neoxanthin (traces) The carotenoid content amounted
to 0.7% of the dry wt. Methods employed included TLC, HPLC, VIS, MS, C
D and H-1 NMR (400 and 500 MHz). The high content of ketocarotenoids i
s characteristic of secondary carotenoids produced under stressed grow
th conditions. Previously secondary carotenoids were associated with g
reen algae (Chlorophyceae), but have now been encountered in Euglenoph
yceae.