GENETIC-STUDIES OF SELF-FERTILITY IN RYE (SECALE-CEREALE L) .1. THE IDENTIFICATION OF GENOTYPES OF SELF-FERTILE LINES FOR THE SF-ALLELES OFSELF-INCOMPATIBILITY GENES

Segregation for self-fertility has been studied in progenies from the crosses of self-sterile (SS) plants with interline hybrids obtained by a diallel scheme of pollinations between seven self-fertile (SF) line s (nos. 2-8) and with F1 (SS plant x SF line) hybrids. All the offspri ng families from the SS plant x F1 (SS plant x SF line) crosses demons trated a 1SF:1SS segregation. The crosses of SS plants with some inter line hybrids gave only self-fertile plants, whereas the crosses with o ther interline hybrids gave a segregation of 3SF:1SS expected in the c ase of digenic segregation. The data obtained permitted us to identify three different S loci (S1, S2, S5) and to estimate the genotypes of self-fertile lines for their Sf alleles: lines 5,6,7 and 8 are S1f/S1f S2n/S2n S5m/S5m, line 4 is S1n/S1n S2f/S2f S5m/S5m, and lines 2 and 3 are S1n/S1n S2m/S2m S5f/S5f (Sn, Sm designate active alleles of the i ncompatibility genes). The identification of the particular S gene whi ch is presented by the Sf allele in each line has been made on the bas is of our data concerning the linkage of the Sf mutation with isozyme markers of particular rye chromosomes, which is reported in an accompa nying paper.