CLEAVAGE OF O-GLYCOSYL LINKAGES IN GLYCOPEPTIDES

Authors
IBATULLIN FM NEUSTROEV KN GOLUBEV AM FIRSOV LM
Citation
Fm. Ibatullin et al., CLEAVAGE OF O-GLYCOSYL LINKAGES IN GLYCOPEPTIDES, Biochemistry, 58(6), 1993, pp. 571-575
Citations number
25
Categorie Soggetti
Biology
Journal title
Biochemistry → ACNP
ISSN journal
00062979
Volume
58
Issue
6
Year of publication
1993
Pages
571 - 575
Database
ISI
SICI code
0006-2979(1993)58:6<571:COOLIG>2.0.ZU;2-X
Abstract
The ability of alpha-mannosidase and alpha-galactosidase to cleave the alpha-linkage between mannose or galactose and serine or threonine wa s studied The following glycopeptides were synthesized as model compou nds simulating the O-glycosyl linkage in glycoproteins: utoxycarbonyl- O-alpha-mannopyranosyl-seryl-glycine methylamide (alpha-Man-Ser-Gly), xycarbonyl-O-alpha-mannopyranosyl-threonyl-glycine methylamide (alpha- Man-Thr-Gly), nosyl-seryl-glycinemethylamide(alpha-Gal-Ser-Gly), and b utoxycarbonyl-O-beta-mannopyranosyl-seryl-glycine methylamide (beta-Ma n-Ser-Gly). The cleavage was shown to occur in glucoamylase after prot eolytic degradation.