Interphase nuclear organization and heterochromatin were studied in si
x species of Crotalaria. All the species showed chromocentric nuclear
organization instead of reticulate organization. The number of chromoc
entres and treatment duration with acid or alkali were found to be spe
cies specific. Percentage heterochromation values determined by two di
fferent techniques were somewhat high in meristematic cells than those
in differentiated cells. On the basis of heterochromatin values in bo
th meristematic and differentiated cells, C. alata may be considered a
s primitive of the six Crotalaria species.