ZETA-COP, A SUBUNIT OF COATOMER, IS REQUIRED FOR COP-COATED VESICLE ASSEMBLY

Authors
KUGE O HARAKUGE S ORCI L RAVAZZOLA M AMHERDT M TANIGAWA G WIELAND FT ROTHMAN JE
Citation
O. Kuge et al., ZETA-COP, A SUBUNIT OF COATOMER, IS REQUIRED FOR COP-COATED VESICLE ASSEMBLY, The Journal of cell biology, 123(6), 1993, pp. 1727-1734
Citations number
31
Categorie Soggetti
Cytology & Histology
Journal title
The Journal of cell biology → ACNP
ISSN journal
00219525
Volume
123
Issue
6
Year of publication
1993
Part
2
Pages
1727 - 1734
Database
ISI
SICI code
0021-9525(1993)123:6<1727:ZASOCI>2.0.ZU;2-E
Abstract
cDNA encoding the 20-kD subunit of coatomer, zeta-COP, predicts a prot ein of 177-amino acid residues, similar in sequence to AP17 and AP19, subunits of the clathrin adaptor complexes. Polyclonal antibody direct ed to zeta-COP blocks the binding of coatomer to Golgi membranes and p revents the assembly of COP-coated vesicles on Golgi cisternae. Unlike other coatomer subunits (beta-, beta', gamma-, and epsilon-COP), zeta -COP exists in both coatomer bound and free pools.