COMPARATIVE-STUDY ON THE EFFECTS OF CHLORITE OXYGEN REACTION-PRODUCT TCDO (TETRACHLORODECAOXYGEN) AND SODIUM-CHLORITE SOLUTION (NACLO2) WITH EQUIMOLAR CHLORITE CONTENT ON BONE-MARROW AND PERIPHERAL-BLOOD OF BDIX RATS

The effects of the chlorite-oxygen reaction product TCDO (tetrachlorod ecaoxygen, active ingredient of the systemic application form of WF 10 ) were investigated on bone marrow and peripheral blood of BDIX rats i n comparison to a sodium chlorite solution with a chlorite content ide ntical to that of WF 10. Despite difficulties in determining the chemi cal differences between TCDO and a sodium chlorite solution, their dif fering effects on cells, tissue and organism were striking. The follow ing characteristics have been observed: Stimulation of the bone marrow , evidenced by the pronounced increase in mature granulocytes, pronorm o- and normoblasts, or increased cell proliferation rate, determined b y means of the BrdUrd method, was achieved only with WF 10 (TCDO). Sti mulation of the bone marrow led in turn to increased numbers of leucoc ytes and monocytes in the peripheral blood. In addition, WF 10 induced the production of large granular lymphocytes (LGLs), referred to as n atural killer cells (NK-cells). in contrast, NaClO2 solution suppresse d bone marrow function, exhibiting a toxic effect when given on a long -term basis. At the same time the number of mature granulocytes as wel l as pronormo- and normoblasts decreased, while tile presence of LGLs was not observed. The results showed that TCDO is a potent stimulator oi the bone marrow function and an effective modulator of the entire i mmune system. The toxic effect of chlorite, derived from the TCDO matr ix, is not completely compensated by the favourable effects of TCDO.