DELAYING EFFECTS OF DIETARY EICOSAPENTAENOIC-DOCOSAHEXAENOIC ACIDS ONDEVELOPMENT OF FATTY STREAKS IN HYPERCHOLESTEROLEMIC RABBITS - A MORPHOLOGICAL-STUDY BY SCANNING ELECTRON-MICROSCOPY
G. Piatti et al., DELAYING EFFECTS OF DIETARY EICOSAPENTAENOIC-DOCOSAHEXAENOIC ACIDS ONDEVELOPMENT OF FATTY STREAKS IN HYPERCHOLESTEROLEMIC RABBITS - A MORPHOLOGICAL-STUDY BY SCANNING ELECTRON-MICROSCOPY, Drugs under experimental and clinical research, 19(4), 1993, pp. 175-181
Besides causing functional and clinical damage, hypercholesterolaemia
also causes morphological alterations of vascular endothelium. Rabbits
fed a diet with 1% cholesterol for 4, 6, or 8 weeks are experimental
models for hypercholesterolaemia, with pathological structural changes
in vascular luminal surface. Morphological investigation by scanning
electron microscopy was performed to reveal the tridimensional growth
of these lesions and the differences in this growth induced by concomi
tant dietary assimilation of fish-oil (2 g/day). Macroscopic reduction
in fatty-streak production was clearly seen in rabbits fed fish-oil.
Scanning electron microscopy confirmed that the area of intimal lesion
s was only 21 +/- 6% in this group, while in the group fed cholesterol
without fish oil, the lesioned area attained 76 +/- 5%. Endothelial s
welling was less marked, probably due to reduced intracellular lipid a
ccumulation into the loam cells. Adherent macrophages were also fewer.
The differences might be correlated with protection against the lipop
roteins' atherogenic effects and to emorheological benefits produced b
y the Omega-3 fatty acid (85%) present in fish-oil.