TRYPANOSOMA-CRUZI GLUTATHIONE-BINDING PROTEINS (TCGBP) - PROTECTION INDUCED BY NATIVE PROTEINS IN AN EXPERIMENTAL-MODEL AND ANALYSIS OF THEANTIBODY-RESPONSE
B. Plumasmarty et al., TRYPANOSOMA-CRUZI GLUTATHIONE-BINDING PROTEINS (TCGBP) - PROTECTION INDUCED BY NATIVE PROTEINS IN AN EXPERIMENTAL-MODEL AND ANALYSIS OF THEANTIBODY-RESPONSE, Research in immunology, 144(8), 1993, pp. 553-563
Three Trypanosoma cruzi glutathione-binding proteins (TcGBP) of 45, 30
and 25 kDa presenting glutathione S transferase activity were charact
erized from T. cruzi epimastigotes. We show here that immunization of
mice using TcGBP and complete Freund's adjuvant (CFA) did not protect
the animals against a challenge with bloodstream trypomastigotes. In c
ontrast, immunization of mice using TcGBP in association with Bordetel
la pertussis plus alum (BpAl) resulted in greatly diminished parasitae
mia and significantly protected the animals from lethal infection. Usi
ng TcGBP mixed with BpAI and a lower challenge dose, we obtained stron
gly diminished parasitaemia and 100 % protection in terms of survival.
Only sera from mice immunized with TcGBP plus BpAl were able to kill
trypomastigotes by complement-mediated lysis, whereas sera from mice i
mmunized with TcGBP plus CFA did not. Interestingly, sera from mice im
munized with TcGBP plus BpAl showed significant levels of specific IgE
, IgG2a and IgG2b antibodies, whereas these isotypes were not detected
in sera from mice immunized with TcGBP in CFA. All these levels were
increased in sera of protected animals. These results demonstrate that
TcGBP antigens can confer resistance to T. cruzi acute infection in m
ice, and suggest a possible functional role for both IgE and IgG2 isot
ypes in the induction of protective immunity.