COUNTS OF ACTIVATED BLOOD EOSINOPHILS FOR MONITORING ASTHMA

Authors
Citation
J. Hed et G. Hallden, COUNTS OF ACTIVATED BLOOD EOSINOPHILS FOR MONITORING ASTHMA, Allergy, 48(17), 1993, pp. 87-93
Citations number
21
Categorie Soggetti
Allergy
Journal title
ISSN journal
01054538
Volume
48
Issue
17
Year of publication
1993
Supplement
S
Pages
87 - 93
Database
ISI
SICI code
0105-4538(1993)48:17<87:COABEF>2.0.ZU;2-2
Abstract
The FOG method is a new cell membrane permeabilization technique which makes it possible to identify the eosinophils in unseparated peripher al blood and allows analyses of both surface and intracellular antigen s by flow cytometry. This technique has been adapted to analyse eosino phil activity in peripheral blood from asthmatic patients by measuring the expression of the EG2-epitope on intracellular ECP.