USE OF SOLID-PHASE PCR FOR ENHANCED DETECTION OF AIRBORNE MICROORGANISMS

Authors
ALVAREZ AJ BUTTNER MP TORANZOS GA DVORSKY EA TORO A HEIKES TB MERTIKASPIFER LE STETZENBACH LD
Citation
Aj. Alvarez et al., USE OF SOLID-PHASE PCR FOR ENHANCED DETECTION OF AIRBORNE MICROORGANISMS, Applied and environmental microbiology, 60(1), 1994, pp. 374-376
Citations number
12
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
60
Issue
1
Year of publication
1994
Pages
374 - 376
Database
ISI
SICI code
0099-2240(1994)60:1<374:UOSPFE>2.0.ZU;2-L
Abstract
The solid-phase PCR (SP-PCR) was compared with a culture-based techniq ue for the detection of aerosolized Escherichia coli DH1. Results with SP-PCR showed an increase in detection sensitivity over that of cultu re methods. Therefore, SP-PCR may be useful for the detection of airbo rne microorganisms which may be nonculturable because of aerosolizatio n or sampling stress.