ISOLATION OF AN INSERTION-SEQUENCE (IS1051) FROM XANTHOMONAS-CAMPESTRIS PV DIEFFENBACHIAE WITH POTENTIAL USE FOR STRAIN IDENTIFICATION AND CHARACTERIZATION

Authors
BERTHIER Y THIERRY D LEMATTRE M GUESDON JL
Citation
Y. Berthier et al., ISOLATION OF AN INSERTION-SEQUENCE (IS1051) FROM XANTHOMONAS-CAMPESTRIS PV DIEFFENBACHIAE WITH POTENTIAL USE FOR STRAIN IDENTIFICATION AND CHARACTERIZATION, Applied and environmental microbiology, 60(1), 1994, pp. 377-384
Citations number
24
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
60
Issue
1
Year of publication
1994
Pages
377 - 384
Database
ISI
SICI code
0099-2240(1994)60:1<377:IOAI(F>2.0.ZU;2-8
Abstract
A new insertion sequence was isolated from Xanthomonas campestris pv. dieffenbachiae. Sequence analysis showed that this element is 1,158 bp long and has 15-bp inverted repeat ends containing two mismatches. Co mparison of this sequence with sequences in data bases revealed signif icant homology with Escherichia coli IS5. IS1051, which detected multi ple restriction fragment length polymorphisms, was used as a probe to characterize strains from the pathovar dieffenbachiae.