A METHOD FOR DETERMINING THE MASS-MOLECULAR COMPOSITION OF MICROBIAL EXOPOLYSACCHARIDES

Authors
VOTSELKO SK PIROG TP MALASHENKO YR GRINBERG TA
Citation
Sk. Votselko et al., A METHOD FOR DETERMINING THE MASS-MOLECULAR COMPOSITION OF MICROBIAL EXOPOLYSACCHARIDES, Journal of microbiological methods, 18(4), 1993, pp. 349-356
Citations number
11
Categorie Soggetti
Microbiology,"Biochemical Research Methods
Journal title
Journal of microbiological methods → ACNP
ISSN journal
01677012
Volume
18
Issue
4
Year of publication
1993
Pages
349 - 356
Database
ISI
SICI code
0167-7012(1993)18:4<349:AMFDTM>2.0.ZU;2-W
Abstract
A method of determining the molecular mass composition of microbial ex opolysaccharides (EPS) by centrifuging them in a combined density grad ient created by NaCl and CsCl solutions and using the molecular mass o f dextranes as standards is developed. The process of determining the molecular mass distribution pattern of EPS is simplified and made cons iderably less time-consuming. This method allows the analysis of nativ e EPS with molecular masses ranging from 13 700 to 2 000 000.