A NOVEL HLA HAPLOTYPE CONTAINING A DRB5 GENE ASSOCIATED WITH THE DRB1-ASTERISK-0103 ALLELE

A new hybrid haplotype, a DRB10103 allele associated with a DRB5*0101 allele, was found in a French Caucasoid family and has been described here. When these cells were typed by serology, contrarily to cells wi th the DR1 + 2s haplotype, they did not seem to be triplets. The react ivity of these cells with the DR2 allosera led to a false serological HLA-DR typing. RFLP analysis and DNA oligotyping after DR1-DRB1, DR2-D RB1 and DRB5 group-specific amplifications showed that there was no DR 2-DRB1 product in these cells and demonstrated the segregation of a DR 103 DR51 haplotype in the family.