The aim of the present clinical trial was to examine the influence of
gingival inflammation on de novo plaque formation. In addition, the ef
fect of a varying number of salivary bacteria on early plaque formatio
n was evaluated. 10 subjects were recruited for the trial which was de
signed to establish 2 different starting points for de novo plaque for
mation, 1 in a healthy dentition and 1 in a gingivitis dentition. On D
ay 0 and at regular intervals during a 5-week period, the panelists we
re examined with respect to gingivitis, plaque and number of salivary
bacteria. The findings from the study demonstrated that the condition
of the marginal gingiva plays an important role in the early stages of
plaque formation. Thus, in both phases of the study, it was observed
that at sites with initially healthy gingival units, less plaque forme
d (PlI = 0.93 and 0.82) than at sites with GI > 0 (PlI = 1.24 and 1.28
) at the 2 different starting points. The number of salivary bacteria
seemed to be less important than the state of the gingiva for the amou
nt of plaque that formed during a 4-day period of no active mechanical
tooth cleaning.