MICROWAVE-ASSISTED STAINING OF MUCOSAL MAST-CELLS AND GRANULATED INTRAEPITHELIAL LYMPHOCYTES AFTER FORMALIN FIXATION

Authors
BIERNACKA D SZYMANSKA K TOMASZEWSKA A GRZELA T MOSKALEWSKI S
Citation
D. Biernacka et al., MICROWAVE-ASSISTED STAINING OF MUCOSAL MAST-CELLS AND GRANULATED INTRAEPITHELIAL LYMPHOCYTES AFTER FORMALIN FIXATION, Histochemical Journal, 25(12), 1993, pp. 854-856
Citations number
21
Categorie Soggetti
Cytology & Histology
Journal title
Histochemical Journal → ACNP
ISSN journal
00182214
Volume
25
Issue
12
Year of publication
1993
Pages
854 - 856
Database
ISI
SICI code
0018-2214(1993)25:12<854:MSOMMA>2.0.ZU;2-R
Abstract
Rat jejunum was fixed with either formalin or methanol-formalin acetic acid (MFAA) and stained with Astra Blue or Alcian Blue with or withou t microwave irradiation. Staining of both mucosal mast cells and granu lated intra-epitheliar lymphocytes after formalin fixation was conside rably improved by microwave irradiation. On the other hand, microwave irradiation slightly impaired staining of mucosal mast cells (MMC) and even more strongly granulated intra-epithelial lymphocytes (GIEL) aft er MFAA fixation.