SPECIFIC AND SENSITIVE DETECTION OF PHYTOPHTHORA-FRAGARIAE VAR RUBI IN RASPBERRY ROOTS BY PCR AMPLIFICATION

Authors
STAMMLER G SEEMULLER E
Citation
G. Stammler et E. Seemuller, SPECIFIC AND SENSITIVE DETECTION OF PHYTOPHTHORA-FRAGARIAE VAR RUBI IN RASPBERRY ROOTS BY PCR AMPLIFICATION, Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz, 100(4), 1993, pp. 394-400
Citations number
14
Categorie Soggetti
Plant Sciences
Journal title
Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz → ACNP
ISSN journal
03408159
Volume
100
Issue
4
Year of publication
1993
Pages
394 - 400
Database
ISI
SICI code
0340-8159(1993)100:4<394:SASDOP>2.0.ZU;2-0
Abstract
A polymerase chain reaction (PCR) method has been developed to detect the raspberry root rot fungus Phytophthora fragariae var. rubi. Primer s were designed to amplify a highly repetitive fragment containing the ribosomal ssu rRNA gene. The procedure is specific for the detection of P. fragariae var. rubi and the closely related strawberry red core pathogen P. fragariae var. fragariae. With the PCR procedure, P. fraga riae var. rubi could be detected in slightly to severely affected rasp berry roots.