HPLC ANALYSIS OF CHIRAL BETA-ADRENERGIC A CTIVE AGENTS

Investigations on direct separation by RP-HPLC of selected enantiomeri c beta-adrenergic active agents are described. R- and S-1-phenylethyli socyanate (PEIC) as well as (1S)-(+)-campher-10-sulfonylchloride (CSC) are used for the derivatization of the compounds. Correlations betwee n chromatographic data and various influences (pH, temperature, reacti on time, concentration of reagens a.o.) have been discussed. The behav iour of derivatized and underivatized analytes on RP-columns is descri bed in dependence on buffer concentration of the mobile phase. The inv estigated beta-adrenergic agents were partly separated after derivatiz ation with PEIC, whereas this was not possible with CSC as derivatizat ion reagent.